Asmaa Ibrahim
Cairo University, Egypt
Biography:
Asmaa Ibrahim is Chemist at Diagnostic and research unit of parasitic diseases (DRUP), Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt
Abstract:
Background and Objectives: The pathogenic bacterium Helicobacter pylori (H.Pylori) and intestinal parasites (IPs) especially Cryptosporidium are well-known for their high prevalence in pediatric patients worldwide especially in developing countries like Egypt. Identifying associations between both infectious agents due to sharing the same mode of infection estimated risks and predictive factors for susceptibility to co-infection.This study conducted to determine the prevalence of H.pylori and Cryptosporidium co-infection in diarrheic Egyptian children by copro PCR restriction fragment length polymorphism (PCR-RFLP), studying their estimated risk and prospective factors.Methodology: Fecal samples were collected from 226 pediatric patients, 125 diarrheic which include both (immunocompetent and immunocompromised) and 101 non diarrheic as a control group. All samples were submitted to coproscopic examination for detection of intestinal parasites before and after concentration, acid-fast stain for coccidian parasites detection such as Cryptosporidium and copro PCR restriction fragment length polymorphism (PCR-RFLP) targeting Cryptosporidium oocyst wall protein (COWP) gene for Cryptosporidium and urease subunit alpha (UreA) gene targeted for H.pylori. Further coproimmunoassay tests were performed only for immunocompromised cases to detect Cryptosporidium and H. pylori coproantigen as a rapid test.Result: Among the studied population positive for giardiasis by microscopy (58%) were co-infected with H. pylori and positive cases for cryptosporidiosis by PCR (70%) also were co-infected with H. pylori. Additionally among the studied variables only immune status (immunocompetent/immunocompromised), diarrhea, vomiting, fever, feeding milk and associated parasites (G. intestinalis and Cryptosporidium) were significantly associated (PË‚0.05) with detection of H.pylori by using multivariate analysis using logistic regression. To our knowledge .No studies demonstrated the co infection between H.pylori and cryptosporidium. Among studied variables immunity status, diarrhea, weight loss, gender and polyparasitism were significantly associated (p<0.01) with Cryptosporidium and H.pylori co- infection.Conclusion: Our results shed light on fundamental role of Intestinal parasites such as Cryptosporidium, Giardia co-infection with H.pylori. Whether H.pylori provides a favorable condition for intestinal parasitosis or vice versa still needs further investigation, emphasis upon determining correlation with gut micro biomes.