Scientific Program

Day 1 :

  • Biochemistry & Stem Cells
Speaker
Biography:

MS. SUCHI is an experienced International Pre School Principal/Manager who picked up  Laughter exercises from many coaches around the world. She then designed ‘Laughter Therapy' which is being used  in many places such as hospitals and Senior Activity Centres. She provides individual and group therapy in educational and home settings. A former Manager / Trainer is now engages in building social awareness about Holistic approach for recovery. Be it Depression,  Anxiety caused by physical or emotional pain,  Death  in the family  and the harm the unhappiness brings to people, families and communities. Her aim is to encourage people to seek help early and get on the path to recovery. Her works has been featured in local press, TV and Radio and has been an invited speaker at various community clubs and educational  Institutions.  She has also been awarded by MINDS and various community clubs  in recognition of her social work.

Abstract:

Statement of the Problem: There is a lack of awareness about what happy hormones are ,how to use positive words to feel energetic and what can be done to get happy hormones. People tend to feel unhappy for multiple reasons and neuropathic pain adds on Stress levels of not only the patient but the caregivers as well. Being in pain leads to feeling depressed and anxious  in some cases.

Methodology & Theoretical Orientation: 

Review of Books and Research shows that getting a dosage of happy  hormones will not only ease slight  pain of the patient but  feeling happy will also have a positive impact on the recovery of the patient. Adopting  Laughter therapy and getting hormones which makes one feel good will help many to recover from Neuropathic pain  /Long term sadness caused by having  grief ,Anger or Resentment, Depression & Anxiety.

Findings: One needs to work on his/her energies using Laughter Therapy which is a  positive approach for not having Depression & Anxiety caused by Neuropathic pain . The therapy can be used as a Holistic way to  recovery. 

Conclusion & Significance: The Laughter therapy which includes ways to get the dosage of happy hormones promotes overcoming Depression & Anxiety caused by Neuropathic pain ,is  a fun way to manage pain.  Repeated sessions to be conducted to remind patients that  life while having pain or during the recovery should go beyond just seeking medical and counselling help and also include rebuilding Spiritual, Physical, Emotional, Relational and Mental health. The model has been put together from for testing in many settings including hospitals ,elderly homes and senior citizen centres.  This is not a research book or paper. It is just an effort to demystify the help available for Depression & Anxiety caused by pain. It is an attempt to motivate and encourage people to seek help and take a simple approach to remember and work on all aspects of their recovery.

Speaker
Biography:

Master degree of analytical chemistry-Faculty of pharmacy-Cairo university. Editorial board member in journal of electrochemical society and many reputable scientific journals. Invited speaker in many international conferences worldwide. Expert in all topics related to analytical chemistry and bioanalysis Since graduation from Faculty of pharmacy-Cairo university May 2006. This lecture is a part of my recent publication " Real-time potentiometric sensor; an innovative tool for monitoring hydrolysis of chemo/bio-degradable drugs in pharmaceutical sciences" published in journal of biomedical and pharmaceutical analysis-The American Chemical Society- February 2018.

Abstract:

Over the last decade, the field of Point-of-care (POC) diagnostics and in vitro diagnostic (IVD) tests have been extensively used and acquired increasing prominence. The outstanding opportunities offered have greatly expanded the application fields and have placed these technologies at the forefront of the tests used in providing life-saving decisions to maintain health, manage disease,  monitor therapy or pre- surgical operation examinations. Being portable and the unique ability of selective, and direct detection of ionic analytes in biological specimens without extraction, are very attractive features of potentiometric Ion Selective Electrodes (ISEs) .The ability to furnish a continuous real time signals allows performing in vitro monitoring of the chemical species in chemical or biological reactions in the real time. Our drug, Atracurium besylate (ATR) is a skeletal muscle relaxant used for anesthesia in surgical operations undergoes chemo-degradation in vivo yielding a toxic metabolite Laudanosine (LDS). A closer insight to the in vivo metabolic processes of ATR, it was reported to be susceptible to degradation by Hofmann elimination as a primary route and ester hydrolysis as a secondary route of chemo-degradation.

Speaker
Biography:

Chanakya N Kundu, professor at School of Biotechnology, KIIT University, Bhubaneswar, completed his PhD in Biochemistry from the Indian Institute of Chemical Biology, Kolkata Jadavpur, India. In prior to join at  KIIT University he worked as a post doc at Texas A&M University, Texas, USA,  Florida Shands Cancer Center, Gainesville, USA and Cleveland Clinic Foundation, Cleveland, Ohio, USA in the field of cancer biology. Currently the focus of his research work is to understand the molecular mechanism of metastasis, angiogenesis in cancer stem cell signaling. He has developed a new chemotherapeutic cocktail which not only inhibits the bulk of cancer cells but also inhibit the cancer stem cell proliferation, angiogenesis as well as reduce the inflammation in cancer patients. He has already published more than 65 peer reviewed research articles in national and international journal, file four patents, multiple book chapter, etc. He is the recipient of multiple award/recognition from the national and international level such as international young investigator award, Dr Kunti and Omprakash oration award, DBT-CREST,etc   He is serving as an editorial board member in multiple journal such as Plos One,  etc.

Abstract:

Although NECTIN-4 ( a pvrl-4 encoded gene) has been reported as a junction protein for decades with recent reports of its advocacy in cancer biology, there remains a paucity of studies exploring its detailed functional significance in metastasis, angiogenesis and cancer stem cells. We report, NECTIN-4 as a probable marker for breast cancer stem cells and provide experimental evidence suggesting that it upregulates WNT/β-CATENIN signaling via PI3K/AKT axis as the self renewal pathway for breast cancer stem cells. Using highly metastatic breast cancer cells and human umbilical vein endothelial cells (HUVECs), we have  also demonstrated the contribution of NECTIN-4 to angiogenesis. We also provide in-depth in ovo, in vitro, in vivo and ex vivo evidence that NECTIN-4 causes metastasis and angiogenesis and propose that nectin-4 is a biomarker both the processes in breast cancer. Following hypoxia, the expression of ADAM-17 in metastatic breast cancer stem cells (mBCSCs) causes the shedding of the ecto-domain of NECTIN-4 into the microenvironment, which physically interacts with integrin-β4 specifically on endothelial cells. This interaction promotes angiogenesis via the Src, PI3K, AKT, iNOS pathway and not by Phospho-Erk or NF-κβ pathways. In vitro, in ovo and in vivo induction and abrogation of an angiogenesis cascade in the presence and absence of the NECTIN-4 ecto-domain, respectively, confirms its role in angiogenesis. Thus, disrupting the interaction between NECTIN-4 ecto-domain and integrin-β4 may provide a means of targeting mBCSC-induced metastasis and angiogenesis.

Speaker
Biography:

Dr noha h.ibrahim has her expertise in evaluation and passion in improving the health and wellbeing. Noha Hassan currently works at the Clinical pathology, National Cancer Institute Egypt. Their most recent publication is 'Canc Therapy & Oncol Int J The Potential Prognostic and Diagnostic Role of MiRNAs as Novel Biomarkers for Prostate Cancer'.

Abstract:

Background: The aim of this work is to study the diagnostic efficacy of mRNA expression of survivin and E2F3 between newly diagnosed patients with prostate cancer, patients with benign prostatic hyperplasia (BPH) and healthy normal control, in addition to comparing them with prostate specific antigen (PSA) that is routinely used in patients with prostate cancer. Also, to study their association with some of the clinicopathological findings of prostate cancer.

Methods: This study included 125 participants; seventy-five patients with prostate adenocarcinoma (PC) presented to the outpatient clinic at the National Cancer Institute, Cairo University over a period of 10 consecutive months from December 2017 to September 2018. Twenty-five patients with BPH and twenty-five, apparently healthy, volunteers were included as benign and normal control groups, respectively. There was detection of survivin and E2F3 mRNA on whole blood samples collected on tubes containing EDTA for all patients and control subjects using semi-quantitative real-time PCR.

Results: Survivin mRNA expression, total PSA, free PSA and PSA ratio showed a statistically significant difference among the control, BPH and PC groups (all have P-value <0.001). On the other hand, E2F3 gave no statistical difference (p=0.272). Survivin is highly expressed in PC than BPH and normal groups and considered as risk factor (OR=1.75, p-value=0.011). survivin and E2F3 has no association with clinicopathological factors of prostate cancer.

Speaker
Biography:

Henry M. Sobell completed his studies at Brooklyn Technical High School (1948-1952), Columbia College (1952-1956), and the University of Virginia School of Medicine (1956-1960). Instead of practicing clinical medicine, he then went to the Massachusetts Institute of Technology (MIT) to join Professor Alexander Rich in the Department of Biology (1960-1965), where, as a Helen Hay Whitney Postdoctoral Fellow, he learned the technique of single crystal X-ray analysis. He then joined the Chemistry Department at the University of Rochester, having been subsequently jointly appointed to both the Chemistry and Molecular Biophysics departments (the latter at the University of Rochester School of Medicine and Dentistry), becoming a full tenured Professor in both departments (1965-1993). He is now retired and living in the Adirondacks in New York, USA.

Abstract:

Premeltons are examples of emergent structures (i.e., structural solitons) that arise spontaneously in DNA due to the presence of nonlinear excitations in its structure. They are of two kinds: B-B (or A-A) premeltons form at specific DNA-regions to nucleate site-specific DNA melting. These are stationary and, being globally nontopological, undergo breather motions that allow drugs and dyes to intercalate into DNA. B-A (or A-B) premeltons, on the other hand, are mobile, and being globally topological, act as phase-boundaries transforming B- into A- DNA during the structural phase-transition. They are not expected to undergo breather-motions. A key feature of both types of premeltons is the presence of an intermediate structural-form in their central regions (proposed as being a transition-state intermediate in DNA-melting and in the B- to A- transition), which differs from either A- or B- DNA. Called beta-DNA, this is both metastable and hyperflexible – and contains an alternating sugar-puckering pattern along the polymer-backbone combined with the partial-unstacking (in its lower energy-forms) of every other base-pair. Beta-DNA is connected to either B- or to A- DNA on either side by boundaries possessing a gradation of nonlinear structural-change, these being called the kink and the antikink regions. The presence of premeltons in DNA leads to a unifying theory to understand much of DNA physical-chemistry and molecular-biology. In particular, premeltons are predicted to define the 5’ and 3’ ends of genes in naked-DNA and DNA in active-chromatin, this having important implications for understanding physical aspects of the initiation, elongation and termination of RNA-synthesis during transcription. For these and other reasons, the model will be of broader interest to the general audience working in these areas. The model explains a wide variety of data, and carries within it a number of experimental predictions – all readily testable – as will be described in my talk.

Speaker
Biography:

Experienced teaching assistant professional with over 2 years of experience working in governmental and private universities, conducting practical courses, Field work. Techniques that I learn from my working as research assistant at institute of endemic Disease gives me enormous ability in conducting research.

Abstract:

BACKGROUND: In this study we analyzed the effect of genetic mutations mainly Single nucleotide polymorphisms (SNPs) mutations that can alter the expression and functions of ATP1A2 gene as gene had been candidate to cause Migraine
 
METHODS: ATP1A2 gene was investigated in dbSNP/NCBI database in June 2016 and we used different computational analysis approach. Deleterious nsSNPs were predicted by SIFT and Polyphen-2 softwares. Then the damaging nsSNPs were submitted to I- mutant to Predict the change in stability due to mutation and PHD-SNP and SNPS&GO software used to demonstrate the relationship between SNP and related Migraine Protein structural analysis of amino acid variants was performed by Project Hope .To highlight genetic interactions of ATP1A2 we used Gene MANIA software.
 
RESULTS: Gena mania revealed that ATP1A2Gene encodes for the α2 subunit of ATPase Na+/ K. The SNPs sequence of ATP1A2 gene was collected from NCBI; 2576of them [Homo sapiens] only From sift and polyphen-2 software the high score deleterious SNPs were found as following: (rs28933401),) rs368405677), (rs121918612), (rs121918614), (rs121918615),( rs121918618), (rs121918619),( rs200425518), (rs181618883)and (rs149144720) Which analysis in Project HOPE software to analyzed the changing in amino acid properties and domains, , which found among these (rs).
(rs28933401),) rs368405677), (rs121918612)have 100% mutation. Additionally, I-Mutant and PHD-SNPs and SNP&GO showed decrease instability for these nsSNP sup on
mutation. Protein structural analysis with these amino acid variants was performed by using I-Mutant, Swiss PDB viewer, to check their molecular dynamics and energy
minimization calculations.
 
CONCLUSION: in this study we found R689Q,T378N,G301R, D718N, P979L, T415M, R171W, A688P,A297T and G855E mutations in ATP1A2gene could directly or indirectly destabilize the amino acid interactions and hydrogen bonds networks thus explaining the functional deviations of protein To some extent mutations in ATP1A2 gene could directly or indirectly destabilize the amino acid interactions and hydrogen bonds networks thus explaining the functional deviations of protein to some extent.

Day 2 :