Scientific Program

Day 1 :

Keynote Forum

Giulio Tarro

President of Foundation de Beaumont Bonelli for cancer research, Naples - Italy

Keynote: From sequence of tumor liberated protein (TLP) to potential targets for diagnosis and therapy

Time : 10:00-10:40AM

Biography:

Giulio Tarro graduated from Medicine School, Naples University (1962). Research Associate, Division of Virology and Cancer Research, Children’s Hospital (1965-1968), Assistant Professor of Research Pediatrics, College Medicine (1968-1969), Cincinnati University, Ohio. Oncological Virology Professor, Naples University (1972-1985). Chief Division Virology (1973-2003), Head Department Diagnostic Laboratories, (2003-2006). D. Cotugno Hospital for Infectious Diseases, Naples; Emeritus, 2006 -. Since 2007 Chairman Committee of Biotechnologies and VirusSphere, World Academy Biomedical Technologies, UNESCO, Adjunct Professor Department Biology, Temple University, College of Science and Technology, Philadelphia, recipient of the Sbarro Health Research Organization lifetime achievement award (2010). His researches have been concerned with the characterization of specific virus-induced tumour antigens, which were the "finger-prints" left behind in human cancer. Achievements include patents in field; discovery of Respiratory Syncytial Virus in infant deaths in Naples and of tumor liberated protein as a tumor associated antigen, 55 kilodalton protein overexpressed in lung tumors and other epithelial adenocarcinomas

Abstract:

A preliminary analysis of immunoprecipitation followed by Western Blotting (WB) shows corin and TLP precipitate at the same level (approximately 50 KDa) and are recognized by the same antibodies. In parallel the tests of immunoprecipitation were improved by the use of cell extracts derived from lung cancer cells A549 and NCI-H23 with the aim of obtaining a precipitate containing only the TLP. In fact the partial amino acid sequence of TLP shows a high homology with the sequence of human corin (only one amino acid is different) and is present in lung cancer under different isoforms. It is known that human corin is expressed mostly outside the cells and the protein extract derived from the extracellular medium and from the cells transfected with the plasmid, which overexpresses corin, shows several bands analysed on SDS-PAGE that are equivalent to the bands (about 50-100 KDa) observed in the WB analysed by anti-TLP. This protein band was identified as aldehyde dehydrogenase isoform 1A1 through mass spectrometry, revealing the molecular nature of at least one component of the previously described TLP complex. Next, we screened a cohort of 29 lung cancer patients (all histologies), 17 patients with non-neoplastic lung pathologies and 9 healthy donors for the presence of serum ALDH1A1 and global serum ALDH by enzyme-linked immunosorbent assay. This analysis indicated that the presence of ALDH was highly restricted to patients with lung cancer. Interestingly, the global ALDH test detected more lung cancer patients compared to the ALDH1A1-specific test, suggesting that other ALDH isoforms might add to the sensitivity of the assay. Our data suggest that ALDH levels may therefore be evaluated as part of a marker panel for lung cancer screening.Finally, the ability of the immune system to recognize a TAA, enables the development of a vaccine approach for preventive and therapeutic application and represents a main target of this field of research.

Keynote Forum

Sergey Suchkov

Chair, Dept for Personalized and Translational Medicine, Royal Society of Chemistry, UK

Keynote: Antibody-Proteases as a Novel Biomarker and a Unique Target to be applied for Biodesign and Bioengineering

Time : 10:40-11:30

Biography:

Sergey Suchkov graduated from Astrakhan State Medical University and awarded with MD, then in 1985 maintained his PhD at the I.M. Sechenov Moscow Medical Academy and in 2001, maintained his Doctorship Degree at the Nat Inst of Immunology, Russia. From 1987 through 1989, he was a senior Researcher, Koltzov Inst of Developmental Biology. From 1989 through 1995, he was a Head of the Lab of Clinical Immunology, Helmholtz Eye Research Institute in Moscow. From 1995 through 2004, a Chair of the Dept for Clinical Immunology, Moscow Clinical Research Institute (MONIKI. Dr Suchkov has been trained at: NIH; Wills Eye Hospital, PA, USA; Univ of Florida in Gainesville; UCSF, S-F, CA, USA; Johns Hopkins University, Baltimore, MD, USA. He was an Exe Secretary-in-Chief of the Editorial Board, Biomedical Science, an international journal published jointly by the USSR Academy of Sciences and the Royal Society of Chemistry, UK. At present, Dr Sergey Suchkov is a Chair, Dept for Personalized and Translational Medicine, I.M.Sechenov First Moscow State Medical University. He is a member of the: New York Academy of Sciences, USA; American Chemical Society (ACS), USA; American Heart Association (AHA), USA; EPMA (European Association for Predictive, Preventive and Personalized Medicine), Brussels, EU; ARVO (American Association for Research in Vision and Ophthalmology); ISER (International Society for Eye Research); PMC (Personalized Medicine Coalition), Washington, USA.

Abstract:

Catalytic Abs (catAbs) are multivalent immunoglobulins (Igs) with a capacity to hydrolyze the antigenic (Ag) substrate. In this sense, proteolytic Abs (Ab-proteases) represent Abs to provide proteolytic effects.Abs against myelin basic protein/MBP with proteolytic activity exhibiting sequence-specific cleavage of MBP are of great value to monitor demyelination whilst in MS. The activity of Ab-proteases was first registered at the subclinical stages 1-2 years prior to the clinical illness. And the activity of the Ab-proteases revealed significant correlation with scales of demyelination and the disability of the patients as well. So, the activity of Ab-proteases and its dynamics tested would confirm a high subclinical and predictive (translational) value of the tools as applicable for personalized monitoring protocols.Of tremendous value are Ab-proteases directly affecting remodeling of tissues with multilevel architectonics (for instance, myelin). By changing sequence specificity one may reach reduction of a density of the negative proteolytic effects within the myelin sheath and thus minimizing scales of demyelination. Ab-proteases can be programmed and re-programmed to suit the needs of the body metabolism or could be designed for the development of new catalysts with no natural counterparts. Further studies are needed to secure artificial or edited Ab-proteases as translational tools of the newest generation to diagnose, to monitor, to control and to treat and rehabilitate MS patients at clinical stages and to prevent the disorder at subclinical stages in persons-at-risks.

Keynote Forum

R Holland Cheng

Finland Distinguished Professor ,University of California, USA

Keynote: Discovery of Structural Modularity for Nanomedicine Design

Time : 11:30-12:15PM

Biography:

R Holland Cheng is Finland Distinguished Professor at University of California, USA
 

Abstract:

Structural proteins of viruses have the capacity to function both in assembly and in disassembly, which is made possible through a built-in flexibility triggered by cellular events.  Focusing on the highly selected viral capsid over their conformational domains between the metastable intermediates and the stable mature forms so far has provided us the essence of the needed stability, resisting extreme pH and digestive enzymes, to deliver medicals or agents to target tumors through both the circulation and the mucosal routes (1-3).  Aided by multimodal imaging integrated with the deep learning via convolutional neural network to guide the tracking and the targeting of the payloads, the design principles of inserting heterologous epitopes to target the mucosal surfaces will be exemplified regarding in this presentation. Deep learning has gained enormous attention by the success of its convolutional neural networks in demonstrated machine learning tasks including high-content image classification.  Crucial precision of cargo deliveries can be better realized through the AI deep-learning and the multiple modality of imaging domains to fully enable the targeting-engineered nanocapsids via various non-invasive mucosal routes. Further advancement of imaging technology like cryoEM and cellular tomography (see details in nobel.se; the Nobel Prize in Chemistry 2017) will be demonstrated in the unveiling of the associated molecular mechanisms essential to the platform vector design towards the success of constructing a non-invasive, mucosal targeting system (4-6).

Keynote Forum

Mark Feitelson

Professor

Keynote: Pharmacotherapy of chronic viral hepatitis and hepatocellular carcinoma

Time : 12:15-01:40PM

Biography:

Mark Feitelson is professor at Temple University, Philadelphia

Abstract:

Statement of the Problem:  Hepatocellular carcinoma (HCC) is the 5th most prevalent tumor type and second leading cause of cancer related deaths worldwide. The most common etiologies associated with HCC are chronic infections with hepatitis B and C viruses.  Although early HCC is curable by surgical resection, it is often asymptomatic and rarely diagnosed in time.  There are many treatment modalities used for patients with advanced HCC, but the efficacy of each approach is limited.  Sorafenib and other multi-kinase inhibitors have been approved for the treatment of advanced HCC, but these only extend life for up to a year.  Alterations in the composition of gut bacteria have been documented in HCC.  Since HCC arises on the background of chronic liver disease (CLD), experiments were carried out to test the hypothesis that the re-establishment of a normal gut microbiome would impact upon the pathogenesis of CLD and HCC.  Methodology: To test this hypothesis, a transgenic mouse model that expressed the hepatitis B oncoprotein, HBx, was used.  This model recapitulates most of the pathology and alterations in many molecular pathways that contribute to human HCC in 100% of the animals within 10-12 months.  Results: When these mice were fed with selected probiotics for several weeks prior to the appearance of dysplastic nodules, half the mice had no evidence of dysplasia, and the rest had smaller nodules and/or small regions of dysplastic cells.  Similar results were observed when mice were treated for several weeks before the appearance of HCC.  More than half the animals had no evidence of HCC, and those with tumors had fewer and smaller nodules.  Conclusions: These observations suggest that manipulation of the gut microbiome delay or block the pathogenesis of CLD and HCC.

  • Biochemistry & Stem Cells

Session Introduction

A. B. Yazykova

Privolzhsky Research Medical University,Russia

Title: Hemoproteins and Elemental Homeostasis in Brain Tumors: Looking Inside the Pathways
Speaker
Biography:

A. B. Yazykova working at Federal State Budgetary Educational Institution of Higher Education “Privolzhsky Research Medical University” of the Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia

Abstract:

The National Center for Biotechnological Information (NCBI) defines bioinformatics as a field of science in which biology, informatics and information technology merge into a single discipline. In bioinformatics there are three important sub-disciplines, such as the development of new algorithms and statistics, through which it is possible to evaluate the relationships between members of large data sets; analysis and interpretation of various types of data, including nucleotide and amino acid sequences, protein domains and protein structures; as well as the development and implementation of tools that provide effective access to and management of various types of information. An effective way to analyse metabolic changes is to build signal networks. The signal network is a probabilistic model of the transmission of a stream of biological information in a cell and between cells, tissues and organs through interactions of cellular agents of different origin. The structural element of the signal network is the binary interaction between two elementary objects. A higher-level component is a signal cascade or path represented by a set of consecutive interactions of partner molecules from the incoming signal to the final reaction associated with the initiation of a specific biological process: activation of gene group expression, opening of ion channels, assembly / disassembly and contraction of the cell cytoskeleton, division, apoptosis etc. The main elements of signalling pathways are proteins, since they are the main regulators of cell activity.

Primary tumors of central nervous system are a rare group of diverse tumors that account for less than 2% of all tumors, but are the fourth most common cause of cancer death. Despite of advances in neurosurgery, the development of intraoperative navigation, as well as chemo and radiation therapy, treatment of primary central nervous system tumors is still insufficiently effective.In the present study, the aim was to investigate blood and tissue macroelements, microelements and hemoproteins level in brain tumors, as well as their intermolecular interactions.

Results and discussion.

Modern databases of signaling pathways (KEGG) suggest that in normal cells the conditions of hypoxia can lead to HIF-1A protein synthesis. Besides, it is known, that chronic intermittent hypoxia (CIH) induces reactive oxygen species (ROS) generation, thereby increasing HIF-1 α expression. Detected reduced magnesium content in brain cancer cells can lead to decreasing of HIF-1 α activation, and probably, can serve as initiating agent for pathologic proliferation development. This factor, together with the protein p53 leads to synthesis and activation of proteins p21/27, which in turn inhibits CDK4/6 and leads to proliferation decrease. Intermittent hypoxia, leading to activation of the NOX enzyme, activates ROS synthesis, which inhibits the PHD enzyme and triggers the release of calcium ions. Calcium ions can be a triggering factor of two fundamental processes, apoptosis (often implemented under normal conditions) and cell proliferation via the Ras protein.  In glioma activation of protein synthesis of EGFR was shown, which, presumably, dramatically increases the release of calcium and thus activates the Ras protein, triggering uncontrolled proliferation. The effects of ROS on the release of calcium ions (Figure 1) may exacerbate this effect. There is evidence that under hypoxic conditions the cell produces myoglobin, which inactivates tumor growth and represents a factor of the cells adaptation to hypoxia. According to the literature a hypothetical relationship with MB is noted, as well as the expression of catalase, reduction of free radicals and oxidative stress

Conclusion.

This is one of the first studies to have examined intermolecular relationships between microelements, hemoproteins and antioxidant enzymes in gliomas. In hypomagnesemia, observed in cancer cells, HIF-1 α stabilization and activation by ROS is violated, and that leads to activation of cell pathological proliferation. Under the circumstances, increased Ca concentration via Ras-way activation leads to cell proliferation activation, more aggravating the situation. Combined disruption of mineral homeostasis and hypoxia, found in the present study, also can serve as factors, stimulating cell proliferation. Established increasing of hemoproteins activity, such as catalase and myoglobin, can possibly be considered as adaptation factor towards hypoxia, oxidative stress and element homeostasis violation.

MS. SUCHI

Chair (Asia ,Global Goodwill Ambassadors), Laughter Coach, Singapore

Title: Adopting laughter therapy to get dosage of happy hormones to remove stress caused by being in slight pain ,being depressed, being unhappy anxious or sad. Saying positive affirmations aloud changes body cell energy
Speaker
Biography:

MS. SUCHI is an experienced International Pre School Principal/Manager who picked up  Laughter exercises from many coaches around the world. She then designed ‘Laughter Therapy' which is being used  in many places such as hospitals and Senior Activity Centres. She provides individual and group therapy in educational and home settings. A former Manager / Trainer is now engages in building social awareness about Holistic approach for recovery. Be it Depression,  Anxiety caused by physical or emotional pain,  Death  in the family  and the harm the unhappiness brings to people, families and communities. Her aim is to encourage people to seek help early and get on the path to recovery. Her works has been featured in local press, TV and Radio and has been an invited speaker at various community clubs and educational  Institutions.  She has also been awarded by MINDS and various community clubs  in recognition of her social work.

Abstract:

Statement of the Problem: There is a lack of awareness about what happy hormones are ,how to use positive words to feel energetic and what can be done to get happy hormones. People tend to feel unhappy for multiple reasons and neuropathic pain adds on Stress levels of not only the patient but the caregivers as well. Being in pain leads to feeling depressed and anxious  in some cases.

Methodology & Theoretical Orientation: 

Review of Books and Research shows that getting a dosage of happy  hormones will not only ease slight  pain of the patient but  feeling happy will also have a positive impact on the recovery of the patient. Adopting  Laughter therapy and getting hormones which makes one feel good will help many to recover from Neuropathic pain  /Long term sadness caused by having  grief ,Anger or Resentment, Depression & Anxiety.

Findings: One needs to work on his/her energies using Laughter Therapy which is a  positive approach for not having Depression & Anxiety caused by Neuropathic pain . The therapy can be used as a Holistic way to  recovery. 

Conclusion & Significance: The Laughter therapy which includes ways to get the dosage of happy hormones promotes overcoming Depression & Anxiety caused by Neuropathic pain ,is  a fun way to manage pain.  Repeated sessions to be conducted to remind patients that  life while having pain or during the recovery should go beyond just seeking medical and counselling help and also include rebuilding Spiritual, Physical, Emotional, Relational and Mental health. The model has been put together from for testing in many settings including hospitals ,elderly homes and senior citizen centres.  This is not a research book or paper. It is just an effort to demystify the help available for Depression & Anxiety caused by pain. It is an attempt to motivate and encourage people to seek help and take a simple approach to remember and work on all aspects of their recovery.

Ahmed Mamun

Cairo university, Egypt

Title: Real-time potentiometric monitoring hydrolysis of a bio-degradable drug (Atracurium besylate)
Speaker
Biography:

Master degree of analytical chemistry-Faculty of pharmacy-Cairo university. Editorial board member in journal of electrochemical society and many reputable scientific journals. Invited speaker in many international conferences worldwide. Expert in all topics related to analytical chemistry and bioanalysis Since graduation from Faculty of pharmacy-Cairo university May 2006. This lecture is a part of my recent publication " Real-time potentiometric sensor; an innovative tool for monitoring hydrolysis of chemo/bio-degradable drugs in pharmaceutical sciences" published in journal of biomedical and pharmaceutical analysis-The American Chemical Society- February 2018.

Abstract:

Over the last decade, the field of Point-of-care (POC) diagnostics and in vitro diagnostic (IVD) tests have been extensively used and acquired increasing prominence. The outstanding opportunities offered have greatly expanded the application fields and have placed these technologies at the forefront of the tests used in providing life-saving decisions to maintain health, manage disease,  monitor therapy or pre- surgical operation examinations. Being portable and the unique ability of selective, and direct detection of ionic analytes in biological specimens without extraction, are very attractive features of potentiometric Ion Selective Electrodes (ISEs) .The ability to furnish a continuous real time signals allows performing in vitro monitoring of the chemical species in chemical or biological reactions in the real time. Our drug, Atracurium besylate (ATR) is a skeletal muscle relaxant used for anesthesia in surgical operations undergoes chemo-degradation in vivo yielding a toxic metabolite Laudanosine (LDS). A closer insight to the in vivo metabolic processes of ATR, it was reported to be susceptible to degradation by Hofmann elimination as a primary route and ester hydrolysis as a secondary route of chemo-degradation.

Chanakya Nath Kundu

Kalinga Institute of Industrial Technology, India

Title: Cell-adhesion protein Nectin-4 (encoded by pvrl-4 gene) induced metastasis and angiogenesis in breast cancer
Speaker
Biography:

Chanakya N Kundu, professor at School of Biotechnology, KIIT University, Bhubaneswar, completed his PhD in Biochemistry from the Indian Institute of Chemical Biology, Kolkata Jadavpur, India. In prior to join at  KIIT University he worked as a post doc at Texas A&M University, Texas, USA,  Florida Shands Cancer Center, Gainesville, USA and Cleveland Clinic Foundation, Cleveland, Ohio, USA in the field of cancer biology. Currently the focus of his research work is to understand the molecular mechanism of metastasis, angiogenesis in cancer stem cell signaling. He has developed a new chemotherapeutic cocktail which not only inhibits the bulk of cancer cells but also inhibit the cancer stem cell proliferation, angiogenesis as well as reduce the inflammation in cancer patients. He has already published more than 65 peer reviewed research articles in national and international journal, file four patents, multiple book chapter, etc. He is the recipient of multiple award/recognition from the national and international level such as international young investigator award, Dr Kunti and Omprakash oration award, DBT-CREST,etc   He is serving as an editorial board member in multiple journal such as Plos One,  etc.

Abstract:

Although NECTIN-4 ( a pvrl-4 encoded gene) has been reported as a junction protein for decades with recent reports of its advocacy in cancer biology, there remains a paucity of studies exploring its detailed functional significance in metastasis, angiogenesis and cancer stem cells. We report, NECTIN-4 as a probable marker for breast cancer stem cells and provide experimental evidence suggesting that it upregulates WNT/β-CATENIN signaling via PI3K/AKT axis as the self renewal pathway for breast cancer stem cells. Using highly metastatic breast cancer cells and human umbilical vein endothelial cells (HUVECs), we have  also demonstrated the contribution of NECTIN-4 to angiogenesis. We also provide in-depth in ovo, in vitro, in vivo and ex vivo evidence that NECTIN-4 causes metastasis and angiogenesis and propose that nectin-4 is a biomarker both the processes in breast cancer. Following hypoxia, the expression of ADAM-17 in metastatic breast cancer stem cells (mBCSCs) causes the shedding of the ecto-domain of NECTIN-4 into the microenvironment, which physically interacts with integrin-β4 specifically on endothelial cells. This interaction promotes angiogenesis via the Src, PI3K, AKT, iNOS pathway and not by Phospho-Erk or NF-κβ pathways. In vitro, in ovo and in vivo induction and abrogation of an angiogenesis cascade in the presence and absence of the NECTIN-4 ecto-domain, respectively, confirms its role in angiogenesis. Thus, disrupting the interaction between NECTIN-4 ecto-domain and integrin-β4 may provide a means of targeting mBCSC-induced metastasis and angiogenesis.

Noha Ibrahim

National Cancer Institute, Egypt

Title: Evaluation of survivin and E2F3 mRNAs as potential diagnostic markers in the blood of prostate cancer patients
Speaker
Biography:

Dr noha h.ibrahim has her expertise in evaluation and passion in improving the health and wellbeing. Noha Hassan currently works at the Clinical pathology, National Cancer Institute Egypt. Their most recent publication is 'Canc Therapy & Oncol Int J The Potential Prognostic and Diagnostic Role of MiRNAs as Novel Biomarkers for Prostate Cancer'.

Abstract:

Background: The aim of this work is to study the diagnostic efficacy of mRNA expression of survivin and E2F3 between newly diagnosed patients with prostate cancer, patients with benign prostatic hyperplasia (BPH) and healthy normal control, in addition to comparing them with prostate specific antigen (PSA) that is routinely used in patients with prostate cancer. Also, to study their association with some of the clinicopathological findings of prostate cancer.

Methods: This study included 125 participants; seventy-five patients with prostate adenocarcinoma (PC) presented to the outpatient clinic at the National Cancer Institute, Cairo University over a period of 10 consecutive months from December 2017 to September 2018. Twenty-five patients with BPH and twenty-five, apparently healthy, volunteers were included as benign and normal control groups, respectively. There was detection of survivin and E2F3 mRNA on whole blood samples collected on tubes containing EDTA for all patients and control subjects using semi-quantitative real-time PCR.

Results: Survivin mRNA expression, total PSA, free PSA and PSA ratio showed a statistically significant difference among the control, BPH and PC groups (all have P-value <0.001). On the other hand, E2F3 gave no statistical difference (p=0.272). Survivin is highly expressed in PC than BPH and normal groups and considered as risk factor (OR=1.75, p-value=0.011). survivin and E2F3 has no association with clinicopathological factors of prostate cancer.

Henry M. Sobell

University of Rochester, USA

Title: The centers of premeltons signal the beginning and ends of genes
Speaker
Biography:

Henry M. Sobell completed his studies at Brooklyn Technical High School (1948-1952), Columbia College (1952-1956), and the University of Virginia School of Medicine (1956-1960). Instead of practicing clinical medicine, he then went to the Massachusetts Institute of Technology (MIT) to join Professor Alexander Rich in the Department of Biology (1960-1965), where, as a Helen Hay Whitney Postdoctoral Fellow, he learned the technique of single crystal X-ray analysis. He then joined the Chemistry Department at the University of Rochester, having been subsequently jointly appointed to both the Chemistry and Molecular Biophysics departments (the latter at the University of Rochester School of Medicine and Dentistry), becoming a full tenured Professor in both departments (1965-1993). He is now retired and living in the Adirondacks in New York, USA.

Abstract:

Premeltons are examples of emergent structures (i.e., structural solitons) that arise spontaneously in DNA due to the presence of nonlinear excitations in its structure. They are of two kinds: B-B (or A-A) premeltons form at specific DNA-regions to nucleate site-specific DNA melting. These are stationary and, being globally nontopological, undergo breather motions that allow drugs and dyes to intercalate into DNA. B-A (or A-B) premeltons, on the other hand, are mobile, and being globally topological, act as phase-boundaries transforming B- into A- DNA during the structural phase-transition. They are not expected to undergo breather-motions. A key feature of both types of premeltons is the presence of an intermediate structural-form in their central regions (proposed as being a transition-state intermediate in DNA-melting and in the B- to A- transition), which differs from either A- or B- DNA. Called beta-DNA, this is both metastable and hyperflexible – and contains an alternating sugar-puckering pattern along the polymer-backbone combined with the partial-unstacking (in its lower energy-forms) of every other base-pair. Beta-DNA is connected to either B- or to A- DNA on either side by boundaries possessing a gradation of nonlinear structural-change, these being called the kink and the antikink regions. The presence of premeltons in DNA leads to a unifying theory to understand much of DNA physical-chemistry and molecular-biology. In particular, premeltons are predicted to define the 5’ and 3’ ends of genes in naked-DNA and DNA in active-chromatin, this having important implications for understanding physical aspects of the initiation, elongation and termination of RNA-synthesis during transcription. For these and other reasons, the model will be of broader interest to the general audience working in these areas. The model explains a wide variety of data, and carries within it a number of experimental predictions – all readily testable – as will be described in my talk.

Afra Mohamed Suliman

Bahri University, Sudan

Title: Computational Analysis of Single Nucleotide Polymorphisms (SNPs) in human ATP1-A2gene
Speaker
Biography:

Experienced teaching assistant professional with over 2 years of experience working in governmental and private universities, conducting practical courses, Field work. Techniques that I learn from my working as research assistant at institute of endemic Disease gives me enormous ability in conducting research.

Abstract:

BACKGROUND: In this study we analyzed the effect of genetic mutations mainly Single nucleotide polymorphisms (SNPs) mutations that can alter the expression and functions of ATP1A2 gene as gene had been candidate to cause Migraine
 
METHODS: ATP1A2 gene was investigated in dbSNP/NCBI database in June 2016 and we used different computational analysis approach. Deleterious nsSNPs were predicted by SIFT and Polyphen-2 softwares. Then the damaging nsSNPs were submitted to I- mutant to Predict the change in stability due to mutation and PHD-SNP and SNPS&GO software used to demonstrate the relationship between SNP and related Migraine Protein structural analysis of amino acid variants was performed by Project Hope .To highlight genetic interactions of ATP1A2 we used Gene MANIA software.
 
RESULTS: Gena mania revealed that ATP1A2Gene encodes for the α2 subunit of ATPase Na+/ K. The SNPs sequence of ATP1A2 gene was collected from NCBI; 2576of them [Homo sapiens] only From sift and polyphen-2 software the high score deleterious SNPs were found as following: (rs28933401),) rs368405677), (rs121918612), (rs121918614), (rs121918615),( rs121918618), (rs121918619),( rs200425518), (rs181618883)and (rs149144720) Which analysis in Project HOPE software to analyzed the changing in amino acid properties and domains, , which found among these (rs).
(rs28933401),) rs368405677), (rs121918612)have 100% mutation. Additionally, I-Mutant and PHD-SNPs and SNP&GO showed decrease instability for these nsSNP sup on
mutation. Protein structural analysis with these amino acid variants was performed by using I-Mutant, Swiss PDB viewer, to check their molecular dynamics and energy
minimization calculations.
 
CONCLUSION: in this study we found R689Q,T378N,G301R, D718N, P979L, T415M, R171W, A688P,A297T and G855E mutations in ATP1A2gene could directly or indirectly destabilize the amino acid interactions and hydrogen bonds networks thus explaining the functional deviations of protein To some extent mutations in ATP1A2 gene could directly or indirectly destabilize the amino acid interactions and hydrogen bonds networks thus explaining the functional deviations of protein to some extent.

Prakash Kinthada

JNTU University, India

Title: Transition metal complexes/organometallic compounds as anticancer/anti HIV drugs or in pharmaceutical industry
Speaker
Biography:

Prakash Kinthada is a Professor in Chemistry at National institute Of Medical Sciences(NIMS) University, Jaipur, RAJASTHAN, INDIA.

Abstract:

Cancer is a dreadful disease and any practical solution in combating this disease is of paramount importance to public health. Cancer patients have burdened by drug induced toxic side effects, and no turned to seek help from the complementary and alternative medicine hoping for a better cure. Research on Platinum based drugs and Non Platinum based drugs is a Multi-Million Dollar Industry in USA and there is every need to produce safe drugs for the cure of this monstrous disease. Flavonoids have a long history of use in traditional medicines in many cultures. The phytochemical, curcumin is one of the major dietary flavonoid, belonging to a group of flavonol, Curcumin is a natural polyphenol. It is highly potential molecule capable of preventing and treating various cancers.  Various dietary chemo preventive agents, turmeric powder or its extract are broadly used as therapeutic preparations in Indian System of medicine. We provide a summarized synthesis and structural determination of Curcumin Oxime, Curcumin Thiosemicarbazone derivative of Gold (III) complex. The use of these analogs for prevention of cancer tumor progression and treatments of human malignancies. A pharmacologic agent for treating and/or preventing cancer, among other diseases and conditions, and particularly breast, prostate, and pancreatic cancer, in humans and animals. The novel pharmacologic agent is an isoflavonoid or isoflavonoid mimetic covalently attached to a cytotoxic pharmacophore that, preferably has the ability to conjugate with a metal salt to form a more potent metal complex, particularly a Au (III) complex and other complexes of Platinum, Palladium, Ruthenium, Copper etc.

Day 2 :